Technology Offer

Ref: 04 SK SKBB 0BHK

Date: 13/12/2004 15:30:06

Title: Monoclonal antibodies

Abstract: A Slovakian institute has developed mouse monoclonal antibodies (MA) prepared by the hybridoma technique. These monoclonal antibodies are able to determine CD molecules (antigen markers) on bovine tissues, single cells as well as on isolated sub-cellular structures. They are open for license and/or joint venture.

Description: A Slovak institute’s proposal involves mouse monoclonal antibodies (MA) prepared by the hybridoma technique. These monoclonal antibodies are able to determine CD molecules (antigen markers) on bovine tissues, single cells as well as on isolated sub-cellular structures. Following hybridoma producing MA against following CD molecules were prepared in this Institute: CD45, CD9, CD41/61, CD11/18, CD62L, and fibrinogen. In addition, they offer other antibodies determining bovine immunoglobulin classes IgG and IgM as well as MA reacting with V-antigen of bovine blood groups. Antibodies can be used in radioimmunological (RIA) tests, enzyme immunological (ELISA) tests and immunofluorescence tests. MAs detecting V-antigens also react in haemolytic test. The majority of commercially available MAs were prepared against human CD molecules. These antibodies usually react only with human cells and are not able to recognize homologous structures in animals. MAs prepared in this institute react with bovine cells and can probably detect homologous structures also in other ruminants. Innovative Aspects: Antibodies can be used in radioimmunological (RIA) tests, enzyme immunological (ELISA) tests and immunofluorescence tests. MAs detecting V-antigens also react in haemolytic test. The majority of commercially available MAs were prepared against human CD molecules. These antibodies usually react only with human cells and are not able to recognise homologous structures in animals. MAs prepared in this institute react with bovine cells and can probably detect homologous structures also in other ruminants. These antibodies usually react only with human cells and are not able to recognize homologous structures in animals. MAs prepared in this institute react with bovine cells and can probably detect homologous structures also in other ruminants.